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recombinant mouse stem cell factor rmscf  (R&D Systems)


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    Structured Review

    R&D Systems recombinant mouse stem cell factor rmscf
    Recombinant Mouse Stem Cell Factor Rmscf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rmscf/Recombinant+Mouse+SCF+Protein/bio_rxiv__2025__03__05__641591-209-36-43
    Average 95 stars, based on 145 article reviews
    recombinant mouse stem cell factor rmscf - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Infection:

    Article Title: IMPDH inhibition activates TLR‐VCAM1 pathway and suppresses the development of MLL‐fusion leukemia
    Article Snippet: To generate sgRNA expression vectors targeting Vcam1 (Vcam1‐knockout‐1 and 2) or nontargeting (NT) control, annealed oligonucleotides were cloned into the lentiGuide‐Puro vector (Addgene #52963; Sanjana et al , ) or pLKO5.sgRNA.EFS.tRFP657 vector (Addgene #57824; Heck et al , ). .. MLL‐AF9 cells were first infected with lentiCas9‐Blast (Addgene #52962; Sanjana et al , ) and were then selected for stable expression of Cas9 in MethocultTM medium (#M3234, STEMCELL Technologies) with 20 ng/ml rmSCF (455‐MC, R&D Systems), 10 ng/ml rmIL‐3 (403‐ML, R&D Systems), 10 ng/ml rmIL‐6 (406‐ML, R&D Systems), 10 ng/ml rmGM‐CSF (415‐ML, R&D Systems) together with blasticidin (10 μg/ml). ..

    Expressing:

    Article Title: IMPDH inhibition activates TLR‐VCAM1 pathway and suppresses the development of MLL‐fusion leukemia
    Article Snippet: To generate sgRNA expression vectors targeting Vcam1 (Vcam1‐knockout‐1 and 2) or nontargeting (NT) control, annealed oligonucleotides were cloned into the lentiGuide‐Puro vector (Addgene #52963; Sanjana et al , ) or pLKO5.sgRNA.EFS.tRFP657 vector (Addgene #57824; Heck et al , ). .. MLL‐AF9 cells were first infected with lentiCas9‐Blast (Addgene #52962; Sanjana et al , ) and were then selected for stable expression of Cas9 in MethocultTM medium (#M3234, STEMCELL Technologies) with 20 ng/ml rmSCF (455‐MC, R&D Systems), 10 ng/ml rmIL‐3 (403‐ML, R&D Systems), 10 ng/ml rmIL‐6 (406‐ML, R&D Systems), 10 ng/ml rmGM‐CSF (415‐ML, R&D Systems) together with blasticidin (10 μg/ml). ..

    Recombinant:

    Article Title: Leptin Receptor, a Surface Marker for a Subset of Highly Engrafting Long-term Functional Hematopoietic Stem Cells
    Article Snippet: .. Freshly sorted cells were plated at 200 cells/ml in 1% methylcellulose culture medium supplemented with 30% FBS (Fisher Scientific), 2 mM L-Glutamine (Lonza), 0.1 mM hemin (Sigma-Aldrich), 50 ng/ml rmSCF (R&D Systems), 1 U/ml recombinant human erythropoietin (rhEPO, Amgen), and 5% vol/vol pokeweed mitogen mouse spleen cell conditioned medium. ..

    Isolation:

    Article Title: Chromatin Remodeling Subunit BRM and Valine Regulate Hematopoietic Stem/Progenitor Cell Function and Self-Renewal Via Intrinsic and Extrinsic Effects
    Article Snippet: .. WT C57BL/6 or Brm −/− Lin − BM cells were isolated and cultured at 50,000 cells/well in mouse HSC expansion media containing RPMI-1640 media (Lonza) with 10% FBS (Fisher Scientific), 100 ng/mL rmSCF (R&D Systems; cat. # 455-MC-010), 100 ng/mL rmTPO (R&D Systems; cat. # 488-TO-200/CF), and 100 ng/mL rmFLT3L (BioLegend; cat. # 550706) with 10 mM L-Valine (Sigma), 10 mM L-Leucine (Sigma) or vehicle control at 37°C at 5% CO 2 and 5% O 2 . ..

    Article Title: Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling
    Article Snippet: .. For expansion of mouse LT-HSCs, Lin – BM cells were isolated as described above, then cultured at 50,000 cells/well in RPMI-1640 media (Lonza) with 10% FBS (Fisher Scientific), 100 ng/ml rmSCF (R&D Systems, catalog 455-MC-010), 100 ng/ml rmTPO (R&D Systems, catalog 488-TO-055/CF), 100 ng/ml rmFlt3L (BioLegend, catalog 550706), and 50 nM rmDEK, 50 nM GST rhDEK, 50 nM GST rhNLS-DEK, 50 nM GST rhDBM-DEK, or vehicle control at 37°C at 5% CO 2 and lowered, 5% O 2 in a humidified chamber. ..

    Cell Culture:

    Article Title: Chromatin Remodeling Subunit BRM and Valine Regulate Hematopoietic Stem/Progenitor Cell Function and Self-Renewal Via Intrinsic and Extrinsic Effects
    Article Snippet: .. WT C57BL/6 or Brm −/− Lin − BM cells were isolated and cultured at 50,000 cells/well in mouse HSC expansion media containing RPMI-1640 media (Lonza) with 10% FBS (Fisher Scientific), 100 ng/mL rmSCF (R&D Systems; cat. # 455-MC-010), 100 ng/mL rmTPO (R&D Systems; cat. # 488-TO-200/CF), and 100 ng/mL rmFLT3L (BioLegend; cat. # 550706) with 10 mM L-Valine (Sigma), 10 mM L-Leucine (Sigma) or vehicle control at 37°C at 5% CO 2 and 5% O 2 . ..

    Article Title: Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling
    Article Snippet: .. For expansion of mouse LT-HSCs, Lin – BM cells were isolated as described above, then cultured at 50,000 cells/well in RPMI-1640 media (Lonza) with 10% FBS (Fisher Scientific), 100 ng/ml rmSCF (R&D Systems, catalog 455-MC-010), 100 ng/ml rmTPO (R&D Systems, catalog 488-TO-055/CF), 100 ng/ml rmFlt3L (BioLegend, catalog 550706), and 50 nM rmDEK, 50 nM GST rhDEK, 50 nM GST rhNLS-DEK, 50 nM GST rhDBM-DEK, or vehicle control at 37°C at 5% CO 2 and lowered, 5% O 2 in a humidified chamber. ..

    Control:

    Article Title: Chromatin Remodeling Subunit BRM and Valine Regulate Hematopoietic Stem/Progenitor Cell Function and Self-Renewal Via Intrinsic and Extrinsic Effects
    Article Snippet: .. WT C57BL/6 or Brm −/− Lin − BM cells were isolated and cultured at 50,000 cells/well in mouse HSC expansion media containing RPMI-1640 media (Lonza) with 10% FBS (Fisher Scientific), 100 ng/mL rmSCF (R&D Systems; cat. # 455-MC-010), 100 ng/mL rmTPO (R&D Systems; cat. # 488-TO-200/CF), and 100 ng/mL rmFLT3L (BioLegend; cat. # 550706) with 10 mM L-Valine (Sigma), 10 mM L-Leucine (Sigma) or vehicle control at 37°C at 5% CO 2 and 5% O 2 . ..

    Article Title: Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling
    Article Snippet: .. For expansion of mouse LT-HSCs, Lin – BM cells were isolated as described above, then cultured at 50,000 cells/well in RPMI-1640 media (Lonza) with 10% FBS (Fisher Scientific), 100 ng/ml rmSCF (R&D Systems, catalog 455-MC-010), 100 ng/ml rmTPO (R&D Systems, catalog 488-TO-055/CF), 100 ng/ml rmFlt3L (BioLegend, catalog 550706), and 50 nM rmDEK, 50 nM GST rhDEK, 50 nM GST rhNLS-DEK, 50 nM GST rhDBM-DEK, or vehicle control at 37°C at 5% CO 2 and lowered, 5% O 2 in a humidified chamber. ..



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